Humanin is a 24-amino acid mitochondrial-derived peptide (MDP) encoded in the mitochondrial genome. It was discovered in 2001 in the brain tissue of Alzheimer's patients. Humanin exhibits cytoprotective, anti-apoptotic, and metabolic-regulating properties, making it a promising peptide for longevity and neuroprotection research.
Cellular Rejuvenation
Anti-aging peptides protect telomeres and activate cellular renewal.
Quickstart Highlights
- Dosage: 1-4mg
- Frequency: Daily or 3x weekly
- Administration: Subcutaneous
- Cycle length: 8-12 weeks
Dosing Plan
| Weeks | Dose | Units |
|---|---|---|
| Weeks 1-2 | 1mg once daily | 20 units (0.2mL) |
| Weeks 3-4 | 2mg once daily | 40 units (0.4mL) |
| Weeks 5+ | 2-4mg once daily or 3x weekly | 40-80 units |
How to mix
2ml BAC water per 10mg vial → 5mg/mL concentration
- Draw 2.0mL bacteriostatic water with a sterile syringe.
- Inject slowly down the vial wall.
- Gently swirl until dissolved.
- Label with date. Concentration = 5mg/mL.
- Refrigerate at 2–8°C. Use within 4 weeks.
Syringe Math Reference
- 10mg vial with 2mL BAC water = 5 mg/mL
- 1mg dose = 20 units (0.2mL)
- 2mg dose = 40 units (0.4mL)
- 4mg dose = 80 units (0.8mL)
What you'll need
Humanin Vials (10mg)
- 4 weeks 2 vials
- 8 weeks 4 vials
- 12 weeks 6 vials
Insulin Syringes (U-100)
- 4 weeks 30 syringes
- 8 weeks 60 syringes
- 12 weeks 90 syringes
Bacteriostatic Water (10mL)
- 12 weeks 2 bottles
Alcohol Swabs
- 12 weeks 1 × 100-count box
How This Works
Humanin is a 24-amino acid peptide encoded in the mitochondrial genome. It binds to multiple receptors including FPRL1, FPRL2, and a trimeric receptor complex (CNTFR/WSX-1/gp130). This binding activates pro-survival pathways (STAT3, PI3K/Akt) and inhibits pro-apoptotic proteins like Bax. Humanin also enhances mitochondrial function, reduces oxidative stress, and improves insulin sensitivity. It crosses the blood-brain barrier and provides neuroprotection against beta-amyloid toxicity.
Mechanism of Action
How Humanin Works in Your Body
Peptide Administered
Distributes to cells throughout the body
Cellular Protection
Activates telomerase and DNA repair pathways
Autophagy Triggered
Old, damaged cells are cleared and recycled
Rejuvenation
Collagen production increases, cells function younger
Benefits & Side Effects
Potential Benefits
- Neuroprotection against beta-amyloid (Alzheimer's relevant)
- Anti-apoptotic effects (cell survival)
- Improved mitochondrial function
- Enhanced insulin sensitivity
- Cardiovascular protection
- Reduced oxidative stress
- Potential longevity benefits
Potential Side Effects
- Limited human safety data
- Injection site reactions (mild)
- Generally well-tolerated in available studies
- Research compound - use with appropriate caution
How to store it
- Lyophilized: Store frozen at -20°C for long-term stability.
- Reconstituted: Refrigerate at 2–8°C; use within 4 weeks.
- Protect from light and heat.
Good to know
- Mitochondrial-derived peptide (MDP) - one of only a few known.
- Discovered in Alzheimer's research - shows neuroprotective properties.
- Works by binding to receptors that regulate apoptosis and metabolism.
- May work synergistically with MOTS-c (another mitochondrial peptide).
- Research is still emerging - primarily preclinical data.
- Generally well-tolerated in available studies.
Community Insights
Summarised from 10 sourcesHumanin is a peptide that affects mitochondria, similar to MOTS-c or SS-31. Reddit users are actively seeking information on its dosage and potential benefits. Discussions often compare its action to other mitochondrial-targeting peptides. Some users have access to Humanin and are planning its use, indicating a growing interest in its therapeutic potential within the community.
Common Dosages Reported
- 25-50ug daily
Stacking Suggestions
- Ipamorelin
- Mod-GRF 1-29 (cjc-1295 no DAC)
- MOTS-c
- SS-31
Reviews & Discussion
Real experiences shared by the community — not medical advice.
Humanin for Anti-Aging and Neuroprotection
A user reported using Humanin periodically for its potential anti-aging and neuroprotective benefits, noting that it seemed to support overall vitality. They mentioned focusing on its role in mitochondrial health and its possible protective effects against age-related cognitive decline.
Mitochondrial-Derived Peptide Interest
One individual expressed interest in Humanin as a longevity regulator, specifically citing its role in mitochondrial-derived signalling. While they did not provide a detailed subjective experience, they highlighted its potential based on emerging research regarding healthspan extension.
Intensive multi-compound cycle including Humanin
A user included Humanin as part of an intensive cycle involving multiple other compounds and peptides. While they reported high energy levels and feeling 'god tier' initially, they noted that their mood and physical state fluctuated significantly throughout the third week, describing the experience as being 'all over the place.'
Intoxicating and Euphoric Effects of Humanin
A user reported that Humanin at a specific dosage was highly intoxicating, providing potent anti-anxiety and euphoric effects. They also noted a sense of very stimulated focus accompanying these emotional shifts.
Research interest in HNG analog
A user expressed interest in running the Humanin analog HNG alongside MOTS-c and SS-31 as part of an anti-aging protocol. They specifically noted HNG as a potent mitochondrial-derived peptide of interest for longevity research.
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